dnasei (New England Biolabs)

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New England Biolabs dnasei
Dnasei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 12565 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dnase+i/DNase+I/pm42892802-60-1-2
Average 99 stars, based on 12565 article reviews

dnasei - by Bioz Stars, 2026-10

99/100 stars

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Incubation:

Article Title: Reverse transcribed ssDNA derepresses translation of a retron antiviral protein.
Article Snippet: Article Reverse transcribed ssDNA derepresses translation of a retron antiviral protein

Purification:

Article Title: Comparison of intracellular and encapsidated viral RNA reveals selective constraints on reovirus recombination
Article Snippet: Total intracellular RNA was isolated using TRIzol Reagent (Invitrogen), according to the manufacturer’s instructions. .. Residual genomic DNA was removed by treatment with RNase-free DNase I (New England Biolabs) at 37°C for 10 minutes, followed by a second RNA purification using TRIzol LS Reagent (Invitrogen). .. Ribosomal RNA was subsequently depleted using the NEBNext rRNA Depletion Kit (New England Biolabs) following the manufacturer’s protocol.

Cell-Signaling:

Article Title: TDP2-mediated DNA repair regulates the dynamics of neuronal early response gene transcription and cortical neuron connectivity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-γH2AX-594 BioLegend Cat#613410; RRID: AB_2616774 Rabbit monoclonal anti-γH2AX (clone EP854(2)Y) Abcam Cat#ab81299; RRID: AB_1640564 Mouse monoclonal anti-γH2AX (clone JBW301) EMD Millipore Cat#05–363; RRID: AB_309864 Rabbit polyclonal anti-H2AX Abcam Cat#ab11175; RRID: AB_297814 Rabbit monoclonal anti-53BP1 (clone EPR2172(2)) Abcam Cat# ab175933; RRID: AB_2890610 Rabbit polyclonal anti-TDP2 Sigma Cat#SAB4301078 Guinea pig polyclonal anti-Cre Recombinase SySy Cat#257–005 Rat monoclonal anti-NeuN-488 (clone W18230A) BioLegend Cat#608456; RRID: AB_3106162 Rabbit monoclonal anti-cFOS (clone Rb108B5) SySy Cat#226–008 Rabbit monoclonal anti-NPAS4 Laboratory of Yingxi Lin N/A Rabbit monoclonal anti-TDP1 (clone D8D1B) Cell Signaling Technologies Cat#59710; RRID: AB_2799572 Alexa-Fluor Secondaries Thermo Fisher Scientific Various Hoechst Thermo Fisher Scientific Cat#H3570 SYBR Gold Thermo Fisher Scientific Cat#S11494 Bacterial and virus strains BAC clone BACPAC Genomic RP24-178I11 BAC clone BACPAC Genomic RP24-368B17 shRNAs (custom) Sigma N/A Chemicals, peptides, and recombinant proteins N-methyl-D-aspartic acid (NMDA) Sigma-Aldrich Cat#M3262 Etoposide (ETP) Tocris Cat#1226 Tetrodotoxin (TTX) Sigma-Aldrich Cat#554412 MG-132 MedChemExpress Cat#HY-13259 PMF01 MedChemExpress Cat#HY-116770 Mirin MedChemExpress Cat#HY-19959 NU7741 MedChemExpress Cat#HY-11006 KU-55933 MedChemExpress Cat#HY-12016 NSC16168 MedChemExpress Cat#HY-100690 FEN1-IN-1 MedChemExpress Cat#HY-123834 Polyethyleneimine (PEI) Sigma-Aldrich Cat#765090 Pierce ChIP-Grade A/G Magnetic Beads Thermo Fisher Scientific Cat#26162 DNase I New England Biolabs CatM0303L Proteinase K New England Biolabs Cat#P8107S Benzonase Nuclease Millipore Sigma Cat#E1014 DNAzol Reagent Thermo Fisher Scientific Cat#10503027 CometAssay Lysis Solution R&D Systems Cat#4250–050–01 Critical commercial assays Lentivirus Titer Kit Abm Cat#LV900 (Continued on next page) 18 Cell Reports 45, 118152, October 27, 2026 ..

Virus:

BAC Assay:

Recombinant:

Chromatin Immunoprecipitation:

Magnetic Beads:

Lysis:

RNA Sequencing:

Article Title: Plasma cell-free RNA ITGB3 is associated with long-term clinical benefit in patients with non-small cell lung cancer receiving immunochemotherapy.
Article Snippet: .. RNA-seq analysis and data processing EV- enriched cfRNA extracted from 1 mL of plasma was treated with DNase I (NEB, Ipswich, Massachusetts, USA) to remove residual DNA. .. Strand- specific RNA sequencing libraries were then prepared using the SMARTer Stranded Total RNA- seq Kit (Clontech, Palo Alto, California, USA).

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